Complete nucleotide sequence of minicircle kinetoplast DNA from Trypanosoma equiperdum.

Sequence heterogeneity in kinetoplast minicircle DNA of Leishmania majer

Complete loss of kinetoplast DNA sequences induced by ethidium bromide or by acriflavine in Trypanosoma equiperdum.

A fully kinetoplastic strain of an antigenic variant of Trypanosoma equiperdum has been made dyskinetoplastic by successive treatments of infected rats with ethidium bromide or acriflavine. After seven passages in the absence of the dye, the loss of kinetoplast DNA (kDNA) is stable and complete as shown by analytical ultracentrifugation, CsCl-dye gradients, and the reassociation kinetics of in ...

sequence heterogeneity in kinetoplast minicircle dna of leishmania majer

0

Structure of Leishmania minicircle kinetoplast DNA classes.

In a recent paper (5), Noyes et al. discussed a nested-PCRbased approach for identification and analysis of Leishmania kinetoplast DNA (kDNA) minicircle classes using the analysis of minicircle variable-region (VR) restriction profiles. The kDNA of trypanosomatids consists of catenated minicircle (approximately 5 3 10 to 5 3 10 per associate) and maxicircle (20 to 50 per associate) molecules (8...

Structure of Leishmania Minicircle Kinetoplast DNA Classes

In a recent paper (5), Noyes et al. discussed a nested-PCRbased approach for identification and analysis of Leishmania kinetoplast DNA (kDNA) minicircle classes using the analysis of minicircle variable-region (VR) restriction profiles. The kDNA of trypanosomatids consists of catenated minicircle (approximately 5 3 10 to 5 3 10 per associate) and maxicircle (20 to 50 per associate) molecules (8...